Anti-phospho-Afadin （Ser1721）抗体是一种脂滴周围主要的相关蛋白，它大量分布在脂质蓄积正常或不正常的细胞中，是脂质蓄积的一个特异性标记物。它在脂肪细胞分化早期就有很高的表达，但当脂肪细胞成熟后，它的表达就明显减少，故称；脂肪分化相关蛋白。

产品编号 yb-3072R
英文名称 Anti-phospho-Afadin (Ser1721)抗体
中文名称 磷酸化丝状肌动蛋白结合蛋白抗体
别    名 p-Afadin(Ser1721); Afadin (phospho S1721); Afadin (phospho Ser1721); MLLT4; Protein AF6; AF6; Afadin; AFAD_HUMAN; ALL1-fused gene from chromosome 6 protein; Protein AF-6.
Anti-phospho-Afadin (Ser1721)抗体  
说 明 书 0.1ml  
产品类型 磷酸化抗体 
研究领域 细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 201kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Afadin around the phosphorylation site of Ser1721 [VL(p-S)PD]
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-phospho-Afadin (Ser1721)抗体
PubMed PubMed
产品介绍 background:
Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12. 

Function:
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

Subunit:
Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.

Subcellular Location:
Cell junction, adherens junction. Note=Not found at cell-matrix AJs.

DISEASE:
Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.

Similarity:
Contains 1 dilute domain.
Contains 1 FHA domain. 
Contains 1 PDZ (DHR) domain. 
Contains 2 Ras-associating domains

Anti-phospho-Afadin (Ser1721)抗体
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.