Anti-CENPB抗体This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix loop

产品编号 yb-6595R
英文名称 Anti-CENPB抗体
中文名称 着丝粒蛋白B抗体
别    名 CENP B; CENP-B; centromere autoantigen B; Centromere protein B; Major centromere autoantigen B; CENPB_HUMAN.
Anti-CENPB抗体 
说 明 书 0.2ml  
研究领域 细胞生物  免疫学  染色质和核信号  细胞周期蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 66kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CENPB (321-360aa)
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix loop helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17 bp sequence (CENPB box) in the centromeric salite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. (referenced from Entrez Gene)

Function:
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid salite DNA, called the CENP-B box. May organize arrays of centromere salite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Subunit:
Homodimer. Interacts with CENPT.

Subcellular Location:
Nucleus. Chromosome, centromere.

Similarity:
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain.

Database links:
UniProtKB/Swiss-Prot: P07199.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.