Anti-CLASP2抗体CLASP2 is a microtubule plus end tracking protein that promotes the stabilization of dynamic microtubules and is required for the polarization of the cytoplasmic

产品编号 yb-7793R
英文名称 Anti-CLASP2抗体
中文名称 细胞连接相关蛋白2抗体
别    名 CLASP 2; CLIP associating protein 2; Cytoplasmic linker associated protein 2; hOrbit2; KIAA0627; Multiple asters (Mast) like homolog 2; Protein Orbit homolog 2; CLAP2_HUMAN.
Anti-CLASP2抗体 
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  信号转导  细胞周期蛋白  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 141kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CLASP2
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
CLASP2 is a microtubule plus end tracking protein that promotes the stabilization of dynamic microtubules and is required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. CLASP2 may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3 kinase signaling. CLASP2 also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Function:
Microtubule plus-end tracking protein that promotes thestabilization of dynamic microtubules. Involved in the nucleationof noncentrosomal microtubules originating from the trans-Golginetwork (TGN). Required for the polarization of the cytoplasmicmicrotubule arrays in migrating cells towards the leading edge ofthe cell. May act at the cell cortex to enhance the frequency ofrescue of depolymerizing microtubules by attaching their plus-endsto cortical platforms composed of ERC1 and PHLDB2. This corticalmicrotubule stabilizing activity is regulated at least in part byphosphatidylinositol 3-kinase signaling. Also performs a similarstabilizing function at the kinetochore which is essential for thebipolar alignment of chromosomes on the mitotic spindle. Acts as amediator of ERBB2-dependent stabilization of microtubules at thecell cortex.

Subunit:
Interacts with CLIP2, ERC1, MAPRE3, microtubules, PHLDB2and RSN. The interaction with ERC1 may be mediated by PHLDB2.Interacts with MAPRE1; probably required for targeting to thegrowing microtubule plus ends. Interacts with GCC2; recruits CLASP2to Golgi membranes. Interacts with MACF1 (By similarity). 

Subcellular Location:
Cytoplasm, cytoskeleton. Cytoplasm,cytoskeleton, centrosome. Chromosome, centromere, kinetochore.Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus,trans-Golgi network. Cell membrane. Cell projection, rufflemembrane. Note=Localizes to microtubule plus ends. Localizes tocentrosomes, kinetochores and the mitotic spindle fromprometaphase. Subsequently localizes to the spindle midzone fromanaphase and to the midbody from ophase. In migrating cellslocalizes to the plus ends of microtubules within the cell body andto the entire microtubule lattice within the lamella. Localizes tothe cell cortex and this requires ERC1 and PHLDB2. TheMEMO1-RHOA-DIAPH1 signaling pathway controls localization of thephosophorylated form to the cell membrane. 

Tissue Specificity:
Brain-specific.

Post-translational modifications:
Phosphorylated by GSK3B. Phosphorylation by GSK3B maynegatively regulate binding to microtubule lattices in lamella.Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the CLASP family.
Contains 5 HEAT repeats. 

Database links:
UniProtKB/Swiss-Prot: O75122.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.