Anti-CLK3抗体Phosphorylates serine- and arginine-rich （SR） proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR

产品编号 yb-7908R
英文名称 Anti-CLK3抗体
中文名称 细胞分裂周期样激酶3抗体
别    名 CDC like kinase 3; CDC-like kinase 3; CLK3; CLK3_HUMAN; Dual specificity protein kinase CLK3.
Anti-CLK3抗体    
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  信号转导  细胞周期蛋白  激酶和磷酸酶  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 73kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from huamn CLK3
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.

Function:
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. 

Subcellular Location:
Isoform 1: Nucleus. Cytoplasm (By similarity). Cytoplasmic vesicle, secretory vesicle, acrosome (By similarity). 
Isoform 2: Nucleus speckle. Note=Co-localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles. 

Tissue Specificity:
Endothelial cells. 

Post-translational modifications:
Autophosphorylates on all three types of residues (By similarity). 

Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 
Contains 1 protein kinase domain. 
atabase links:
UniProtKB/Swiss-Prot: P49761.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.