Anti-CART抗体The CART gene encodes for a protein which has an important role in the regulation of appetite and body weight. The CART （cocaine- and amphetamine-regulated

产品编号 yb-12145R
英文名称 Anti-CART抗体
中文名称 调节转录蛋白抗体
别    名 CART; CART prepropeptide; Cocaine and amphetamine regulated transcript; CART_HUMAN.
Anti-CART抗体
说 明 书 0.2ml  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  转录调节因子  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 10kDa
细胞定位 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CART
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
The CART gene encodes for a protein which has an important role in the regulation of appetite and body weight. The CART (cocaine- and amphetamine-regulated transcript) neuropeptide is an mRNA that changes in response to psychostimulant drug administration. Injection of CART peptides into the ventral tegmental area produces psychostimulant-like effects. CART localizes to areas of the central and peripheral nervous systems and is involved in feeding behavior when injected centrally. Expression of CART in the rat hypothalamus is modulated by nutritional status, and injection of synthetic CART peptide into the forebrain ventricular system suppresses food intake, indicating a possible role in hypothalamic control of energy homeostasis. Its identification in cell bodies and central terminals of vagal afferent neurons additionally suggests a role in brainstem mechanisms of meal termination and satiety.

Function:
CART (Cocaine and amphetamine regulated transcript protein) is implicated in reward, feeding, and stress, and it has the functional properties of an endogenous psychostimulant. It is closely associated with the actions of leptin and neuropeptide Y. It promotes neuronal development and survival in vitro.

Subcellular Location:
Secreted

Tissue Specificity:
Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus. 

Similarity:
Belongs to the CART family. 

Database links:
UniProtKB/Swiss-Prot: Q16568.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.