Anti-Cingulin抗体Diffusion of solutes is prevented across certain barriers by the formation of tight junction seals. Occludin and Cingulin interact with other proteins to

产品编号 yb-13956R
英文名称 Anti-Cingulin抗体
中文名称 扣带蛋白抗体
别    名 6330408J11Rik; AI647528; AI987749; cgn; CING_HUMAN; Cingulin; DKFZp779N1112; FLJ39281; KIAA1319; MGC118157.
 Anti-Cingulin抗体
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  信号转导  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 136kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cingulin
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
Diffusion of solutes is prevented across certain barriers by the formation of tight junction seals. Occludin and Cingulin interact with other proteins to direct the formation and regulation of tight junctions. Cingulin, a protein component of the submembrane plaque of tight junctions (TJ), contains globular and coiled-coil domains and interacts in vitro with several TJ and cytoskeletal proteins, including the PDZ protein ZO-1. Cingulin binding has also been shown to inhibit RhoA activation and signaling with increased Cingulin expression in confluent cells, causing downregulation of RhoA by inhibiting GEF-H1/Lfc.

Function:
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular Location:
Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.

Tissue Specificity:
Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
 
Similarity:
Belongs to the cingulin family.