Anti-CYB5R2抗体NADH-cytochrome b5 reductases participate in a variety of physiological processes including biosynthesis of cholesterol,

产品编号 byb-14131R
英文名称Anti-CYB5R2抗体
中文名称 细胞色素B5还原酶2抗体
别    名 b5R.2; Cyb5r2; Cytochrome b5 reductase 2; Cytochrome b5 reductase b5R.2; NADH cytochrome b5 reductase 2; NADH-cytochrome b5 reductase 2; NB5R2_HUMAN.
Anti-CYB5R2抗体 
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 31kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CYB5R2
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
NADH-cytochrome b5 reductases participate in a variety of physiological processes including biosynthesis of cholesterol, methemoglobin reduction of erythrocytes, elongation of fatty acids and metabolism of drugs. CYB5R2 (cytochrome b5 reductase 2), also known as B5R.2, is a 276 amino acid protein that contains one FAD-binding FR-type domain and belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Existing as two alternatively spliced isoforms, CYB5R2 reduces 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt and lucigenin in spermatozoa, which results in NADH-dependent lucigenin chemiluminescence. CYB5R2 binds FAD as a cofactor and is encoded by a gene located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

Function:
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

Tissue Specificity:
Restricted expression.

Similarity:
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.