Anti-DOCK2抗体The DOCK2 gene encodes dedicator of cytokinesis 2 （DOCK 2）, a hematopoietic cell-specific CDM family

产品编号 yb-12431R
英文名称 Anti-DOCK2抗体
中文名称 细胞质分裂付出蛋白2抗体
别    名 Dedicator of cyto kinesis 2; Dedicator of cytokinesis 2; Dedicator of cytokinesis protein 2; DOCK 2; FLJ46592; KIAA0209; DOCK2_HUMAN.
 Anti-DOCK2抗体   
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  细胞生物  信号转导  干细胞  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 212kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DOCK2
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
The DOCK2 gene encodes dedicator of cytokinesis 2 (DOCK 2), a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis. DOCK 2 participates in the cytoskeletal rearrangements that are required for lymphocyte migration in response of chemokines. This peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK 2 may also participate in IL-2 transcriptional activation through the activation of Rac 2. DOCK 2 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain. The DHR-2 domain is a putative GEF activity mediator. The DOCK 2 protein also co-localizes with F-Actin, and demonstrates expression in several human tissues, with the highest levels observed in peripheral blood leukocytes, thymus, spleen and liver. 

Function:
DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.

Subunit:
Homodimer (Probable). Interacts with RAC1 and RAC2. Interacts with CRKL and VAV. Interacts with CD3Z. 

Subcellular Location:
Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
 
Tissue Specificity:
Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon.