AntiDAPP1抗体B cell adapter molecule is also designated dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

产品编号 yb-12982R
英文名称 AntiDAPP1抗体
中文名称 B淋巴细胞衔接分子DAPP1抗体
别    名 B cell adapter molecule of 32 kDa; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa; BAM32; DAPP1; DAPP1_HUMAN; DKFZp667E0716; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; Dual adaptor of phosphotyrosine and 3 phosphoinositides; hDAPP1.
 AntiDAPP1抗体
说 明 书 0.2ml  
研究领域 心血管  细胞生物  神经生物学  淋巴细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Horse, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 32kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DAPP1/BAM32
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
B cell adapter molecule is also designated dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC g2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.

Function:
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Subunit:
Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2. 

Subcellular Location:
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Tissue Specificity:
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Contains 1 PH domain.
Contains 1 SH2 domain.

Database links:
UniProtKB/Swiss-Prot: Q9UN19.1
 
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.