Anti-DZIP3抗体Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction.

产品编号 yb-13044R
英文名称 Anti-DZIP3抗体
中文名称 E3泛素蛋白连接酶dzip3抗体
别    名 DAZ-interacting protein 3; E3 ubiquitin-protein ligase DZIP3; hRUL138; KIAA0675; RNA-binding ubiquitin ligase of 138 kDa; DZIP3_HUMAN.
Anti-DZIP3抗体    
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  表观遗传学  泛素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Recombinant Fragment
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 139kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human DZIP3
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). DZIP3 (DAZ interacting protein 3, zinc finger), also known as UURF2 or hRUL138, is a 1,208 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Expressed in a variety of tissues with highest expression in heart, skeletal muscle and kidney, DZIP3 functions as an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme, thereby playing a role in signaling events throughout the cell. Multiple isoforms of DZIP3 exist due to alternative splicing events.

Function:
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.

Subunit:
Interacts with DAZ proteins.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Widely expressed at low level. Highlyexpressed in skeletal muscle, kidney and heart. Expressed at lowlevel in placenta, lung, brain, liver and pancreas.

Similarity:
Contains 1 RING-type zinc finger. 

Database links:
UniProtKB/Swiss-Prot: Q86Y13.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.