Anti-ENC1抗体Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells.

产品编号 yb-8043R
英文名称Anti-ENC1抗体
中文名称 外胚层神经皮层蛋白1/KLHL35抗体
别    名 CCL 28; CCL28; Ectoderm neural cortex protein 1; Ectoderm-neural cortex protein 1; Ectodermal neural cortex (with BTB like domain); ENC 1; ENC-1; ENC1; ENC1_HUMAN; FLJ39259; Kelch like 35; Kelch like 37; Kelch-like protein 37; KLHL 35; KLHL35; KLHL37; NRPB; Nuclear matrix protein NRP/B; Nuclear restricted protein BTB domain like (brain); p53 induced gene 10 protein; p53-induced gene 10 protein; PIG 10; PIG10; TP53I10; Tumor protein p53 inducible protein 10.
Anti-ENC1抗体  
说 明 书 0.2ml  
研究领域 细胞生物  免疫学  神经生物学  细胞类型标志物  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 66kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ENC1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificity: Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.

Function:
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 

Subunit:
Binds to RB1. Hypophosphorylated RB1 associates with ENC1 during neuronal differentiation, while hyperphosphorylated RB1 associates with ENC1 in undifferentiating cells. Part of a complex that contains CUL3, RBX1 and ENC1. 

Subcellular Location:
Nucleus matrix. Cytoplasm, cytoskeleton. 

Tissue Specificity:
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. 

Post-translational modifications:
Ubiquitinated and probably targeted for proteasome-independent degradation.