Anti-EPS15抗体Elucidation of the mechanism by which receptor tyrosine kinases （RTKs） modulate cellular physiology in response to stimuli is critical

产品编号 yb-11251R
英文名称 Anti-EPS15抗体
中文名称 胞吞调控蛋白Eps15抗体
别    名 AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15; EPS15_HUMAN.
Anti-EPS15抗体 
说 明 书 0.2ml  
研究领域 肿瘤  信号转导  生长因子和激素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, 
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 99kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human EPS15
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimay in cancer. Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15, have been described. Eps8 and Eps15 are proteins, respectively that become tyrosine phosphorylated subsequent to EGF stimulation. Overexpression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement in the regulation of cell proliferation. Eps15 is capable of binding the amino terminal portion of Crk via a conserved proline-rich domain, characteristic of all Crk binding proteins (5). Overexpression of Eps8 in both fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF. Eps8 has been shown to associate with the EGF receptor despite its lack of a functional SH2 domain. Further characterization suggests the protein has both a PH domain and a SH3 domain, the functional significance of which are not yet known.

Function:
The eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis