Anti-ERC2抗体ERCs （ERC1 and ERC2） also referred to as ELKS and CAST are related proteins which share an identical C-terminal sequence.

产品编号yb-11345R
英文名称 Anti-ERC2抗体
中文名称 CAST1蛋白抗体
别    名 Cast; CAST1; CAZ-associated structural protein 1; Cmbp (gene name); Cytomatrix protein p110; ERC protein 2; KIAA0378; ERC2_HUMAN.
Anti-ERC2抗体
说 明 书 0.1ml  0.2ml  
研究领域 细胞生物  神经生物学  信号转导  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 110kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CAST1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
ERCs (ERC1 and ERC2) also referred to as ELKS and CAST are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. ERC2/CAST 1 is only expressed as a single RIM binding variant. All ERCs have been shown to interact with Rab6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.

Function:
Thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ. 

Subunit:
Interacts with BSN, ERC1, PPFIA1, PPFIA2, PPFIA3 and PPFIA4. Interacts through its C-terminus with the PDZ domain of RIMS1. Part of a complex consisting of ERC2, RIMS1 and UNC13A. 

Subcellular Location:
Cytoplasm. Cell junction; synapse. Cell junction; synapse: synaptosome. Cytoskeleton. Note: In neurons, localized to synapses, and colocalizes with PCLO. Localized to the active zone of presynaptic density.


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.