Anti-EVI5抗体EVI5 is an 810 amino acid protein that contains one Rab-GAP TBC domain and exists in both monomeric and dimeric form.

产品编号 yb-14649R
英文名称 Anti-EVI5抗体
中文名称 神经母细胞瘤4S基因抗体
别    名 ecotropic viral integration site 5; NB4S; EVI5_HUMAN; Neuroblastoma stage 4S gene.
 Anti-EVI5抗体  
说 明 书 0.2ml  
研究领域 细胞生物  神经生物学  细胞周期蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 93kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human EVI5
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 
PubMed PubMed
产品介绍 background:
EVI5 is an 810 amino acid protein that contains one Rab-GAP TBC domain and exists in both monomeric and dimeric form. Localizing to the nucleus and cytoplasm, EVI5 is expressed in various cell lines, as well as in brain and adrenal tissue. EVI5 acts as an important regulator of cell cycle progression by stabilizing Emi1 and promoting cyclin-A accumulation, and may also play a role in cytokinesis. EVI5 interacts with alpha- and gamma-tubulin and the chromosome passenger complex (CPC), and undergoes phosphorylation and ubiquitination. EVI5 degradation during prophase is ubiquitin dependent, while phosphorylation is required for degradation during mitosis. The gene encoding EVI5 maps to human chromosome 1p22.1 and mouse chromosome 5 F. Depletion of EVI5 as a result of RNA interference results in cell cycle arrest and mitotic abnormalities. EVI5 may also be a potential at-risk gene for multiple sclerosis (MS).
 
Function:
EVI5 is a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics. It may act as an oncogene.