Anti-Cullin 2抗体Cullin 2 is a member of the family of human Cullin genes （CUL1, 2, 3, 4a, 4b and 5） homologous to the S. cerevisiae cdc53 gene.

产品编号 yb-4209R
英文名称 Anti-Cullin 2抗体
中文名称 Cullin2抗体
别    名 Cullin2; Cullin-2; CUL 2; MGC131970; CUL2_HUMAN.
nti-Cullin 2抗体  
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  细胞生物  免疫学  染色质和核信号  信号转导  细胞周期蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 87kDa
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cullin 2
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
nti-Cullin 2抗体
PubMed PubMed
产品介绍 background:
Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Function:
Core component of multiple cullin-RING-based ECS(ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligasecomplexes, which mediate the ubiquitination of target proteins. Mayserve as a rigid scaffold in the complex and may contribute tocatalysis through positioning of the substrate and theubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligaseactivity of the complex is dependent on the neddylation of thecullin subunit and is inhibited by the association of thedeneddylated cullin subunit with TIP120A/CAND1 (By similarity). Thefunctional specificity of the ECS complex depends on the substraterecognition component. ECS(VHL) mediates the ubiquitination ofhypoxia-inducible factor (HIF).

Subunit:
Component of multiple ECS (Elongin BC-CUL2/5-SOCS-boxprotein) E3 ubiquitin-protein ligase complexes formed of CUL2,Elongin BC (TCEB1 and TCEB2), RBX1 and a variablesubstrate-specific adapter. Component of the ECS(VHL) or CBC(VHL)complex containing VHL. Component of the ECS(MED8) complex with theprobable substrate recognition component MED8 (By similarity).Component of the ECS(LRR1) complex with the probable substraterecognition component LRR1. Component of a probable ECS E3ubiquitin-protein ligase complex containing CUL2, RBX1, TCEB1,TCEB2 and FEM1B. Part of an E3 ubiquitin-protein ligase complexincluding ZYG11B, CUL2 and Elongin BC. Part of an E3ubiquitin-protein ligase complex including ZYG11BL, CUL2 andElongin BC. Interacts with RBX1, RNF7, FEM1B and TIP120A/CAND1.Interacts with COPS2, and MED8 (By similarity). Interacts withhuman respiratory syncytial virus (HRSV) protein NS1. Found in acomplex composed of LIMD1, VHL, EGLN1/PHD2, TCEB2 AND CUL2.
nti-Cullin 2抗体
Similarity:
Belongs to the cullin family.

Database links:
UniProtKB/Swiss-Prot: Q13617.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.